Antigens and antibodies have been extensively validated and characterized. The antigens were analyzed by UV–Vis spectroscopy and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The antibody sensitivity was determined by measuring the 50% inhibition concentration (IC50) by icELISA. The antibody specificity was determined by measuring cross-reactivity (CR) with other structural analogs. The result shows that the IC50 of RHA™ anti-Enrofloxacin monoclonal antibody, clone ENOR was 0.0303 ng/mL. https://www.creative-diagnostics.com/food-analysis/tag-enrofloxacin.html