Gene expression and therapy is the introduction of foreign nucleic acids into target cells to modify or compensate for gene defects, so as to treat diseases such as cancer, diabetes, autoimmune diseases and infectious diseases. The rate of removal of exposed nucleic acid is faster, and the rate of non-specific biological distribution and cell internalization is low, which cannot achieve the expected therapeutic effect. Non-viral vectors have low immunity, can be used for multiple injections and mass production, and have great potential for clinical gene therapy. Common non-viral vector transgenic methods include electroporation, calcium phosphate precipitation, and liposomes. https://www.cd-bioparticles.net/gene-expression