A fully validated dissolution in vitro release method is one of the control tools to ensure that quality and clinical performance are maintained throughout the lifecycle of the drug products. For example, in vitro release methods may aid in the characterization of liposome integrity, and in quantifying free versus encapsulated drugs. Like drug products without nanomaterials, drug products containing nanomaterials should have dissolution/in vitro release methods capable of discriminating formulation and manufacturing differences which may impact the clinical performance of the drug product. In addition to in vitro drug release, drug in vitro testing also includes in vitro toxicity test, in vitro drug sensitivity tests and so on. https://www.cd-bioparticles.net/drug-in-vitro-testing