Autophagy is an orderly circulatory system within a cell that can degrade unnecessary cytoplasmic proteins and damaged organelles. It is a dynamic autophagy process that exists in all eukaryotic cells. Autophagy is important for cells to resist intracellular and extracellular stress and maintain metabolic homeostasis. It is involved in many physiological events, including cell renewal, cell growth, immunity and aging. Recent evidence indicates that changes in autophagy often occur in many human diseases, such as neurodegenerative diseases, cancer, and cardiomyopathy. Therefore, finding new drugs that target the autophagy pathway is essential for the treatment of human diseases. https://www.bioimagingtech.com/autophagy-imaging-analysis.html