The indirect targeting of mRNA is essential to translating ribosome affinity purification (TRAP). By attaching an affinity tag, such as enhanced GFP (EGFP), to the large ribosomal subunit protein L10a, indirect labeling of mRNA can be accomplished. The EGFP-L10a transgene is targeted to express in the desired cell type using the right genetic components. While translating polysomes from targeted cells have EGFP tags, those from non-targeted cells do not.
For more: https://ribosome.creative-biolabs.com/translating-ribosome-affinity-purification-trap.htm

Only people mentioned by @creativebiolabs in this post can reply

No replys yet!

It seems that this publication does not yet have any comments. In order to respond to this publication from creativebiolabs , click on at the bottom under it