Cas9-C-NLS nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein with a C-terminal nucleic localization signal (NLS). It can be used for genome editing by inducing site-specific DNA double stranded breaks. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the NLS. https://molecular-tools.creative-enzymes.com/cas9-c-nls-nuclease-item-279.html